Pnm-3: The Effect of Ginger Hydro-Alcohol Essence on The Ovarian Hormons in the Brest Cancer Rats by Treatment Cyclophosphamide in Estrus Cycle

Background: Cyclophosphamid as an anti-neoplastic medicine, is classified as a derivative of mustard nitrogen, which passes through brain- blood -barrier and it is known as causing significant poisoning affects with high stoppage effects on body. Through attaching two DNA chains of cell nucleus and breaking inter/intra-chain connection and inhibition RNA synthesis Cyclophosphamide causes high toxicity effects on body. Of main effect of the medicine is Amenorrhea, reducing hypothalamuses- hypophysis gonad hormones, ovaries fibrosis, infertility, arthritis, Thrombophlebitis, renal toxicity, bladder fibrosis, and cystic hemorrhagic. Materials and Methods: The study is conducted over 40 rats of Wistar race with average weight of 45 g. in 5 group each of which with 8 members. First, samples were excited by IP injection of PMSG (10 IU) in follicular phase, then ovulation activated by administering HCG (10 IU), and then we injected to them 3 mg/Kg/daily DMBA in via gavage process and they received Brest cancer after 5 weeks. Control group received no medicine. While sham group only received medicine solvent and experiment group received cyclophosphamide with a dose of 5 mg/Kg/BW. After 21 days samples received ginger hydro-alcohol essence (gavage) with dose of 1 g/ kg/BW with Cyclophosphamide. Results: During this study, statistical tests kruskal-wallis on different concentrations of hypophysis gonadotropic plasma hormones estrogen, progesterone and different factors were examined. Rates in experimental group which received hydro-alcohol essences with Cyclophosphamid placed at p<0.05 level in comparison to the group which only received Cyclophosphamid and there was a significant increase in gonadotropic hormones. Conclusion: This research showed us that ginger hydro- alcohol essence with dose of 1 g/kg/BW could inhibit pharmacology effects of Cyclophosphamide for fertility.